ISOLATION, BIOCHEMICAL CHARACTERIZATION AND ANTIBIOTIC SENSITIVITY OF LACTOBACILLI IN QUAIL DROPPINGS

Abstract

Background: Probiotics are microorganisms  which are when given in a specific amount, have favorable effects on the host health. Objectives: The current study was conducted on quail’s droppings, which were collected from the various sailing points within district Dera Ismail Khan. The core aim of this field study was to isolate the Lactobacillus species from the droppings to identify and characterize them. Methods: Three species (lactobacillus brevis, lactobacillus salivarius and lactobacillus fermentum) were isolated on the basis of biochemical tests. Results: Lactobacillus salivarius was found more tolerant at pH 2 as compared to the other two species of lactobacillus, likewise at pH 3 lactobacillus brevis showed maximum growth, which was significantly higher than other species of lactobacillus. Similarly, all the three species of lactobacillus showed higher growth on 0.5 % ox gall as compared to the 1 % ox gall. Likewise antibiotic sensitivity testing of lactobacillus species shown sensitivity to erythromycin except L. fermentum and all three were resistance to tetracycline, kanamycin, ciprofloxacin and gentamycin. Conclusion: It is concluded that lactobacillus species have huge benefits like their tolerance to low pH and their growth on bile salts. Their use  in diets will not only balance the gut microflora but also will inhibit the growth of pathogens in humans, animals and poultry species, as  described by different studies which are conducted on above mentioned species.