• Muhammad Shoaib khan Department of Companion Animal Medicine and Surgery, Faculty of Veterinary Medicine, Universiti Putra Malaysia; Faculty of Veterinary and animal Sciences, Gomal University D.I.Khan, Pakistan
  • Mohd Basyaruddin Abdul Rahman Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia
  • Adamu Abdul Abubakar Department of Veterinary Medicine, College of Applied and Health Sciences, A Sharqiyah University, Oman
  • Shakeeb Ullah Faculty of Veterinary and Animal Sciences, Gomal University DIKhan, Pakistan
  • Humaira A. Khan Department of Veterinary Internal Diseases, University Of Erciyes Turkiye
  • Farheen Bhatti Department of Bioinformatics & Biotechnology, Government College University Faisalabad, Pakistan
  • Nayab Batool Rizvi School of chemistry Punjab University, Lahore, Pakistan
  • Sadaf Shakoor University of Agriculture Faisalabad Sub Campus Burewala-Vehar
  • Muhammad Inamullah Malik Faculty of Veterinary and Animal Sciences, Gomal University, DIKhan, Pakistan
  • Loqman Mohammad Yusof Department of Companion Animal Medicine and Surgery, Faculty of Veterinary Medicine, Universiti Putra Malaysia
  • Saifur Rehman Gomal University Dera Ismail Khan



Background: Scientists and physicians have long faced challenges in their quest to identify the most effective methods for cryopreservation of living tissues and organs. While cryopreservation techniques have been used since the 1800s, they are still used for tissue preservation for a comparatively limited time. More advancements in biotechnology for tissue and organ preservation have been made possible by the availability of commercially available liquefied gases. Typical issues such as sub-zero or cell damage and re-crystallization, which ultimately results in tissue loss, impede the usefulness of cryopreservation in the field of medical sciences. However, modern cryobiology advances have provided many alternatives for chilling tissues, organs, gametes, and embryos. As tissue sensitivity to ice is more understood, safer freezing methods are used. Methods: This review discusses how tissue cryopreservation advances biotechnology. The effective banking and transplanting of living tissues and organs using cryopreservation will be a crucial and noteworthy development in the fields of biotechnology and medicine in the future. One of the most crucial elements that will enable future cryobiologists and biotechnology researchers to make enormous strides is the preservation and storage of living tissues from the point of donor removal through transfer and transplantation. Future cryobiologists will find this ancient tale fascinating as it unfolds, thanks to the discovery of anti-freezing proteins and peptides in cryopreservation's active zones.